The constituents of few Blepharis species have been studied. 9-Hydroxydodecanoic acid has been isolated from the seed oil of Blepharis sindica (Ahmad et al., 1983).
The seeds of Blepharis sindica contained allantoin (90), betaine (91), β-sitosterol, oleanolic acid, apigenin, terniflorin (92), purine-6″-O-coumarate (93) and blepharin (54) (Ahmad et al., 1984).
Allantoin was also identified in Blepharis sindica stalks (Khatri et al., 1989). Daniel and Sabnis (1987) identified luteolin and apignin 7-O-methyl ether from Blepharis madaraspatensis (L.) Heyne, in addition to apigenin 7-O-methyl ether and apigenin-4′-Omethyl ether from Blepharis repens Roth. Phenolic acids have been identified in some Blepharis species e.g. p-hydroxybenzoic, α-resorcylic and vanillic acids from Blepharis asperima Nees; p-hydroxybenzoic, 2-hydroxy-4-methoxybenzoic, vanillic and syringic acids from Blepharis madaraspatensis, and genistic, protocatechuic and syringic acids from Blepharis repens Rothy (Daniel and Sabnis, 1987).